Search results for " electrochemical detection"
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Development of an Analytical Assay for Electrochemical Detection and Quantification of Protein-Bound 3-Nitrotyrosine in Biological Samples and Compar…
2020
Reactive oxygen and nitrogen species (RONS) cause oxidative damage, which is associated with endothelial dysfunction and cardiovascular disease, but may also contribute to redox signaling. Therefore, their precise detection is important for the evaluation of disease mechanisms. Here, we compared three different methods for the detection of 3-nitrotyrosine (3-NT), a marker of nitro-oxidative stress, in biological samples. Nitrated proteins were generated by incubation with peroxynitrite or 3-morpholino sydnonimine (Sin-1) and subjected to total hydrolysis using pronase, a mixture of different proteases. The 3-NT was then separated by high performance liquid chromatography (HPLC) and quantifi…
NiO@Ni core shell for electrochemical detection of Hg ions
2018
Heavy metals are considered as one of the most dangerous chemicals for human health. Among heavy metals, mercury is one of the most dangerous one: few ppb of Hg can cause serious damages to brain, heart, lungs and kidney. The Environmental Protection Agency setted the maximum concentration of Hg in water as 2 ppb [1] . Nowadays is very important to develop new and novel sensors for heavy metals that could be cheap, easy to use and for in-situ and real-time analysis and in this context electrochemical sensors are perfect candidates [2] . The more powerful electrochemical tequinique is Square Wave Anodic Strippic Voltammetry (SWASV), a two step techinique were the heavy metals are first depos…